Histochemical Analysis of Expression Pattern of Camv 35S Promoter in Transgenic Rapeseed (Brassica Napus L.)
نویسندگان
چکیده
منابع مشابه
regeneration and transformation of oilseed (brassica napus) using camv 35s promoter- β-glucuronidase gene
camv 35s promoter activity has not been analysed in different stages of growth in oil-seed (b. napus). higher plants lack intrinsic β-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. the binary transformation vector pvw432 (buchanan-wollaston, unpublished), carrying the gus gene fused to the camv 35s promoter, was used to test transformation efficienci...
متن کاملAgrobacterium–mediated Transformation and Regeneration of Fertile Transgenic Plants of Rapeseed (Brassica napus L.)
A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...
متن کاملWhole-Genome Identification and Expression Pattern of the Vicinal Oxygen Chelate Family in Rapeseed (Brassica napus L.)
Vicinal oxygen chelate proteins (VOC) are members of the metalloenzyme superfamily, which plays roles in many biological reactions. Some members of the VOC superfamily have been systematically characterized but not in Brassica napus. In this study, 38 VOC genes were identified based on their conserved domains. The present results revealed that most of the BnaVOC genes have few introns, and all ...
متن کاملCombining Ability Analysis of Yield Component Parameters in Winter Rapeseed Genotypes (Brassica napus L.)
Eight winter rapeseed genotypes GA096, Geronimo, Okapi, Orient, Sunday, Zarfam, SW0756, and Modena were used as parents of a complete diallel mating design. A set of 56 diallel F1 hybrids (with reciprocals) including their parents were evaluated in a 8×8 simple lattice design with two replications during 2007/08. Several agronomic traits like Plant height, number of lateral branches per pod, nu...
متن کاملبررسی پروموتور CaMV 35S با استفاده از ژن گزارشگرGUS در کلزای (Brassica napus) تراریخت
نظر به اینکه بخش اعظم روغن خوراکی کشور (بیش از 90%) از خارج تأمین میگردد، لذا افزایش تولید دانههای روغنی به منظور قطع وابستگی کشور ضرورت دارد. از بین نباتات روغنی، کلزا از اهمیت خاصی برخوردار است و اصلاح برای کمیت و کیفیت روغن آن لازم است. با توجه به اینکه اصلاح گیاه روغنی کلزا با استفاده از روشهای کلاسیک وقتگیر، پرهزینه و گاهی غیر ممکن است، در نتیجه استفاده از تکنیکهای نوین بیوتکنولوژی و ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current World Environment
سال: 2015
ISSN: 0973-4929,2320-8031
DOI: 10.12944/cwe.10.special-issue1.90